Gene:  HTT, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Huntingtin is a disease gene linked to Huntington's disease a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded unstable trinucleotide repeat in the huntingtin gene which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1 highly expressed in brain which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: HTT, Human
huntingtin

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0048191
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055451
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: HTT, Human
huntingtin

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0013484
List Price:    $125.00
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PrimePCR™ Probe Assay: HTT, Human
huntingtin

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0055451
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: HTT, Human
huntingtin

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5012447
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0048191; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: HTT, Human
huntingtin

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5019357
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0013484; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: HTT, Human
huntingtin

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5043204
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0055451; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: HTT, Human
huntingtin

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5043205
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0055451; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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