Gene:  HSD17B10, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids alcohols and steroids. The protein has been implicated in the development of Alzheimer's disease and mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified but the full-length nature of only two transcript variants has been determined. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: HSD17B10, Human
hydroxysteroid (17-beta) dehydrogenase 10

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042322
Info:   Same primer pair as used in probe assay qHsaCEP0049585
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PrimePCR™ SYBR® Green Assay: HSD17B10, Human
hydroxysteroid (17-beta) dehydrogenase 10

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0013850
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PrimePCR™ Probe Assay: HSD17B10, Human
hydroxysteroid (17-beta) dehydrogenase 10

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049585
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: HSD17B10, Human
hydroxysteroid (17-beta) dehydrogenase 10

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5007264
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0042322; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: HSD17B10, Human
hydroxysteroid (17-beta) dehydrogenase 10

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5019640
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0013850; Intron-spanning
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PrimePCR™ ddPCR™ Copy Number Assay: HSD17B10, Human
hydroxysteroid (17-beta) dehydrogenase 10

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500253
Info:   FAM; 68nt amplicon; exonic; FAM version of HSD17B10 (dHsaCP2506387)
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Copy Number Assay: HSD17B10, Human
hydroxysteroid (17-beta) dehydrogenase 10

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506387
Info:   HEX; 68nt amplicon; exonic; HEX version of HSD17B10 (dHsaCP2500253)
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Expression Probe Assay: HSD17B10, Human
hydroxysteroid (17-beta) dehydrogenase 10

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5032840
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0049585; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: HSD17B10, Human
hydroxysteroid (17-beta) dehydrogenase 10

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5032841
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0049585; exonic
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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