Gene:  HLA-DRB1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB) both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes dendritic cells macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3 DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3 DRB4 and DRB5. There are 4 related pseudogenes: DRB2 DRB6 DRB7 DRB8 and DRB9. [provided by RefSeq Jul 2008]

PrimePCR™ SYBR® Green Assay: HLA-DRB1, Human
major histocompatibility complex, class II, DR beta 1

Assay Type: SYBR® Green
Assay Design: intron_spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0037245
Info:   Same primer pair as used in probe assay qHsaCIP0039968
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PrimePCR™ Probe Assay: HLA-DRB1, Human
major histocompatibility complex, class II, DR beta 1

Assay Type: Probe
Assay Design: intron_spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0039968
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LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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