Gene:  HLA-B, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide exon 2 and 3 encode the alpha1 and alpha2 domains which both bind the peptide exon 4 encodes the alpha3 domain exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: HLA-B, Human
major histocompatibility complex, class I, B

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0037470
Info:   Same primer pair as used in probe assay qHsaCEP0040193
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PrimePCR™ Probe Assay: HLA-B, Human
major histocompatibility complex, class I, B

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0040193
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PrimePCR™ ddPCR™ Mutation Assay:HLA-B p.A329V, Human
major histocompatibility complex, class I, B

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504586
Info:   64nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:HLA-B WT for p.A329V, Human
major histocompatibility complex, class I, B

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504587
Info:   64nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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