Gene:  HIF1A, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1) which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes including those involved in energy metabolism angiogenesis apoptosis and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq Jul 2011]

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PrimePCR™ SYBR® Green Assay: HIF1A, Human
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042813
Info:   Same primer pair as used in probe assay qHsaCEP0050075
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PrimePCR™ SYBR® Green Assay: HIF1A, Human
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0014755
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: HIF1A, Human
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050075
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PrimePCR™ ddPCR™ Mutation Assay:HIF1A p.K213Q, Human
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500822
Info:   69nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:HIF1A WT for p.K213Q, Human
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500823
Info:   69nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:HIF1A p.D238N, Human
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503300
Info:   67nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:HIF1A WT for p.D238N, Human
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503301
Info:   67nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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