Gene:  GTF2H2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This gene is within the telomeric copy of the duplication. Deletion of this gene sometimes accompanies deletion of the neighboring SMN1 gene in spinal muscular atrophy (SMA) patients but it is unclear if deletion of this gene contributes to the SMA phenotype. This gene encodes the 44 kDa subunit of RNA polymerase II transcription initiation factor IIH which is involved in basal transcription and nucleotide excision repair. Transcript variants for this gene have been described but their full length nature has not been determined. A second copy of this gene within the centromeric copy of the duplication has been described in the literature. It is reported to be different by either two or four base pairs; however no sequence data is currently available for the centromeric copy of the gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: GTF2H2, Human
general transcription factor IIH, polypeptide 2, 44kDa

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0038711
Info:   Same primer pair as used in probe assay qHsaCIP0041433
List Price:    $125.00
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PrimePCR™ Probe Assay: GTF2H2, Human
general transcription factor IIH, polypeptide 2, 44kDa

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0041433
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: GTF2H2, Human
general transcription factor IIH, polypeptide 2, 44kDa

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5190008
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0038711; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: GTF2H2, Human
general transcription factor IIH, polypeptide 2, 44kDa

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5193415
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0041433; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: GTF2H2, Human
general transcription factor IIH, polypeptide 2, 44kDa

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5193416
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0041433; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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