Gene:  GRIA2, Human

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Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) and function as ligand-activated cation channels. These channels are assembled from 4 related subunits GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing resulting in transcript variants encoding different isoforms (including the flip and flop isoforms that vary in their signal transduction properties) has been noted for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: GRIA2, Human
glutamate receptor, ionotropic, AMPA 2

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0056529
Info:   Same primer pair as used in probe assay qHsaCEP0057634
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PrimePCR™ SYBR® Green Assay: GRIA2, Human
glutamate receptor, ionotropic, AMPA 2

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0009010
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: GRIA2, Human
glutamate receptor, ionotropic, AMPA 2

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0057634
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: GRIA2, Human
glutamate receptor, ionotropic, AMPA 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5013218
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0056529; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: GRIA2, Human
glutamate receptor, ionotropic, AMPA 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5016064
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0009010; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: GRIA2, Human
glutamate receptor, ionotropic, AMPA 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5044746
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0057634; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: GRIA2, Human
glutamate receptor, ionotropic, AMPA 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5044747
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0057634; exonic
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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