Gene:  GHRL, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes ghrelin-obestatin preproprotein which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety improving memory regulating sleep affecting cell proliferation and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression. [provided by RefSeq Oct 2008]

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PrimePCR™ SYBR® Green Assay: GHRL, Human
ghrelin/obestatin prepropeptide

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0036675
Info:   Same primer pair as used in probe assay qHsaCEP0039399
List Price:    $125.00
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PrimePCR™ Probe Assay: GHRL, Human
ghrelin/obestatin prepropeptide

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0039399
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: GHRL, Human
ghrelin/obestatin prepropeptide

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5005839
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0036675; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: GHRL, Human
ghrelin/obestatin prepropeptide

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5029990
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0039399; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: GHRL, Human
ghrelin/obestatin prepropeptide

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5029991
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0039399; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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