Gene:  GHR, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the type I cytokine receptor family which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome also known as the growth hormone insensitivity syndrome (GHIS) a disorder characterized by short stature. In humans and rabbits but not rodents growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq Jun 2011]

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PrimePCR™ SYBR® Green Assay: GHR, Human
growth hormone receptor

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043464
Info:   Same primer pair as used in probe assay qHsaCEP0050726
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PrimePCR™ SYBR® Green Assay: GHR, Human
growth hormone receptor

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015051
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: GHR, Human
growth hormone receptor

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050726
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: GHR, Human
growth hormone receptor

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5008232
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0043464; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: GHR, Human
growth hormone receptor

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5020505
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0015051; Intron-spanning
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PrimePCR™ ddPCR™ Copy Number Assay: GHR, Human
growth hormone receptor

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506274
Info:   FAM; 68nt amplicon; crosses exon-intron junction; FAM version of GHR Exon 3 (dHsaCP2506691)
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Copy Number Assay: GHR, Human
growth hormone receptor

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506691
Info:   HEX; 68nt amplicon; crosses exon-intron junction; HEX version of GHR Exon 3 (dHsaCP2506274)
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Expression Probe Assay: GHR, Human
growth hormone receptor

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5034776
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0050726; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: GHR, Human
growth hormone receptor

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5034777
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0050726; exonic
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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