Gene:  FLT3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains one transmembrane region and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis proliferation and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: FLT3, Human
fms-related tyrosine kinase 3

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043927
Info:   Same primer pair as used in probe assay qHsaCEP0051189
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PrimePCR™ SYBR® Green Assay: FLT3, Human
fms-related tyrosine kinase 3

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0021972
Info:   Recommended - best efficiency
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PrimePCR™ Probe Assay: FLT3, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051189
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PrimePCR™ ddPCR™ Copy Number Assay: FLT3, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000582
Info:   69nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: FLT3 p.D835Y, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000047
Info:   67nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: FLT3 WT for p.D835Y, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000048
Info:   67nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: FLT3 p.D835H, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500492
Info:   68nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: FLT3 WT for p.D835H, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500493
Info:   68nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 p.D835V, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501972
Info:   68nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 WT for p.D835V, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501973
Info:   68nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 p.G831E, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502522
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 WT for p.G831E, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502523
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 p.L561L, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503534
Info:   82nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 WT for p.L561L, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503535
Info:   82nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 p.V557A, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503864
Info:   75nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 WT for p.V557A, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503865
Info:   75nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 p.A627T, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504068
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 WT for p.A627T, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504069
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 p.I836M, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504430
Info:   68nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 WT for p.I836M, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504431
Info:   68nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 p.D835N, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504588
Info:   68nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 WT for p.D835N, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504589
Info:   68nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 p.D835E, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504724
Info:   68nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 WT for p.D835E, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504725
Info:   68nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 p.D835E, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505618
Info:   68nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FLT3 WT for p.D835E, Human
fms-related tyrosine kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505619
Info:   68nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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