Gene:  FLG, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
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The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor profilaggrin (consisting of multiple filaggrin units of 324 aa each) which is localized in keratohyalin granules and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq Dec 2009]

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PrimePCR™ SYBR® Green Assay: FLG, Human
filaggrin

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0036604
Info:   Same primer pair as used in probe assay qHsaCEP0039328
List Price:    $125.00
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PrimePCR™ Probe Assay: FLG, Human
filaggrin

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0039328
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: FLG, Human
filaggrin

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5005797
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0036604; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: FLG, Human
filaggrin

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5029906
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0039328; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: FLG, Human
filaggrin

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5029907
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0039328; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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