Gene:  FGFR3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the fibroblast growth factor receptor (FGFR) family with its amino acid sequence being highly conserved between members and among divergent species. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region composed of three immunoglobulin-like domains a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors setting in motion a cascade of downstream signals ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene lead to craniosynostosis and multiple types of skeletal dysplasia. Three alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq Jul 2009]

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PrimePCR™ SYBR® Green Assay: FGFR3, Human
fibroblast growth factor receptor 3

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0004573
List Price:    $130.00
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PrimePCR™ SYBR® Green Assay: FGFR3, Human
fibroblast growth factor receptor 3

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042267
Info:   Same primer pair as used in probe assay qHsaCEP0049530
List Price:    $130.00
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PrimePCR™ Probe Assay: FGFR3, Human
fibroblast growth factor receptor 3

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049530
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: FGFR3, Human
fibroblast growth factor receptor 3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5002765
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0004573; Exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: FGFR3, Human
fibroblast growth factor receptor 3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5007214
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0042267; exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Copy Number Assay: FGFR3, Human
fibroblast growth factor receptor 3

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000503
Info:   FAM; 69nt amplicon; crosses exon-intron junction; FAM version of FGFR3 (dHsaCP2506340)
 
Fluorophore:   FAM
List Price:    $240.00
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PrimePCR™ ddPCR™ Mutation Assay: FGFR3 p.Y373C, Human
fibroblast growth factor receptor 3

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000103
Info:   75nt amplicon; exonic
 
Fluorophore:   FAM
List Price:    $395.00
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PrimePCR™ ddPCR™ Mutation Assay: FGFR3 WT for p.Y373C, Human
fibroblast growth factor receptor 3

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000104
Info:   75nt amplicon; exonic
 
Fluorophore:   HEX
List Price:    $395.00
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PrimePCR™ ddPCR™ Mutation Assay: FGFR3 p.G380R, Human
fibroblast growth factor receptor 3

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500516
Info:   60nt amplicon; exonic
 
Fluorophore:   FAM
List Price:    $395.00
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PrimePCR™ ddPCR™ Mutation Assay: FGFR3 WT for p.G380R, Human
fibroblast growth factor receptor 3

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500517
Info:   60nt amplicon; exonic
 
Fluorophore:   HEX
List Price:    $395.00
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PrimePCR™ ddPCR™ Copy Number Assay: FGFR3, Human
fibroblast growth factor receptor 3

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506340
Info:   HEX; 69nt amplicon; crosses exon-intron junction; HEX version of FGFR3 (dHsaCP1000503)
 
Fluorophore:   HEX
List Price:    $240.00
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PrimePCR™ ddPCR™ Mutation Assay: FGFR3 p.S249C, Human
fibroblast growth factor receptor 3

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506760
Info:   57nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
List Price:    $395.00
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PrimePCR™ ddPCR™ Mutation Assay: FGFR3 WT for p.S249C, Human
fibroblast growth factor receptor 3

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506761
Info:   57nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
List Price:    $395.00
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PrimePCR™ ddPCR™ Mutation Assay: FGFR3 p.R248C, Human
fibroblast growth factor receptor 3

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506906
Info:   57nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
List Price:    $395.00
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PrimePCR™ ddPCR™ Mutation Assay: FGFR3 WT for p.R248C, Human
fibroblast growth factor receptor 3

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506907
Info:   57nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
List Price:    $395.00
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PrimePCR™ ddPCR™ Expression Probe Assay: FGFR3, Human
fibroblast growth factor receptor 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5032740
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0049530; exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: FGFR3, Human
fibroblast growth factor receptor 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5032741
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0049530; exonic
 
Fluorophore:   HEX
List Price:    $195.00
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PrimePCR™ ddPCR™ Mutation Assay:FGFR3 p.K650M, Human
fibroblast growth factor receptor 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501098
Info:   65nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
List Price:    $325.00
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PrimePCR™ ddPCR™ Mutation Assay:FGFR3 WT for p.K650M, Human
fibroblast growth factor receptor 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501099
Info:   65nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
List Price:    $325.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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