Gene:  FGFR2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a member of the fibroblast growth factor receptor family where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region composed of three immunoglobulin-like domains a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors setting in motion a cascade of downstream signals ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic basic and/or keratinocyte growth factor depending on the isoform. Mutations in this gene are associated with Crouzon syndrome Pfeiffer syndrome Craniosynostosis Apert syndrome Jackson-Weiss syndrome Beare-Stevenson cutis gyrata syndrome Saethre-Chotzen syndrome and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq Jan 2009]

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PrimePCR™ SYBR® Green Assay: FGFR2, Human
fibroblast growth factor receptor 2

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0021928
Info:   Same primer pair as used in probe assay qHsaCIP0033242
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PrimePCR™ Probe Assay: FGFR2, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0033242
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PrimePCR™ ddPCR™ Copy Number Assay: FGFR2, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000023
Info:   91nt amplicon; intronic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: FGFR2 p.S252W, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000045
Info:   63nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: FGFR2 WT for p.S252W, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000046
Info:   63nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Copy Number Assay: FGFR2, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500320
Info:   63nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FGFR2 p.C382R, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502112
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FGFR2 WT for p.C382R, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502113
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FGFR2 p.K659E, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502294
Info:   85nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FGFR2 WT for p.K659E, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502295
Info:   85nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FGFR2 p.N549K, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503248
Info:   80nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FGFR2 WT for p.N549K, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503249
Info:   80nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FGFR2 p.P253R, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503836
Info:   63nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FGFR2 WT for p.P253R, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503837
Info:   63nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FGFR2 p.Y375C, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504794
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FGFR2 WT for p.Y375C, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504795
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FGFR2 p.N549K, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505356
Info:   80nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FGFR2 WT for p.N549K, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505357
Info:   80nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FGFR2 p.E636E, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2506164
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FGFR2 WT for p.E636E, Human
fibroblast growth factor receptor 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2506165
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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