Gene:  FGFR1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region composed of three immunoglobulin-like domains a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors setting in motion a cascade of downstream signals ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome Jackson-Weiss syndrome Antley-Bixler syndrome osteoglophonic dysplasia and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however not all variants have been fully characterized. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: FGFR1, Human
fibroblast growth factor receptor 1

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0001617
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PrimePCR™ SYBR® Green Assay: FGFR1, Human
fibroblast growth factor receptor 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042405
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049667
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PrimePCR™ Probe Assay: FGFR1, Human
fibroblast growth factor receptor 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049667
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PrimePCR™ ddPCR™ Copy Number Assay: FGFR1, Human
fibroblast growth factor receptor 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000022
Info:   79nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Copy Number Assay: FGFR1, Human
fibroblast growth factor receptor 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500319
Info:   69nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FGFR1 p.T141A, Human
fibroblast growth factor receptor 1

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505094
Info:   98nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FGFR1 WT for p.T141A, Human
fibroblast growth factor receptor 1

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505095
Info:   98nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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