Gene:  F8, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes coagulation factor VIII which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which in the presence of Ca+2 and phospholipids converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein isoform a which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein isoform b which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A a common recessive X-linked coagulation disorder. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: F8, Human
coagulation factor VIII, procoagulant component

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0017878
Info:   Same primer pair as used in probe assay qHsaCIP0031517
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PrimePCR™ Probe Assay: F8, Human
coagulation factor VIII, procoagulant component

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0031517
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PrimePCR™ ddPCR™ Mutation Assay:F8 p.R1715R, Human
coagulation factor VIII, procoagulant component

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2506068
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:F8 WT for p.R1715R, Human
coagulation factor VIII, procoagulant component

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2506069
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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