Gene:  EZH2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein the VAV1 oncoprotein and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Feb 2011]

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PrimePCR™ SYBR® Green Assay: EZH2, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043150
Info:   Same primer pair as used in probe assay qHsaCEP0050412
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PrimePCR™ SYBR® Green Assay: EZH2, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0011970
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PrimePCR™ Probe Assay: EZH2, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050412
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PrimePCR™ ddPCR™ Copy Number Assay: EZH2, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000508
Info:   69nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 p.E249K, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501686
Info:   66nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 WT for p.E249K, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501687
Info:   66nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 p.Y646N, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501772
Info:   69nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 WT for p.Y646N, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501773
Info:   69nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 p.Y646F, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502000
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 WT for p.Y646F, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502001
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 p.Y646S, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502342
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 WT for p.Y646S, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502343
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 p.R690H, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502414
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 WT for p.R690H, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502415
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 p.Y646C, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503294
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 WT for p.Y646C, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503295
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 p.Y646H, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504380
Info:   69nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 WT for p.Y646H, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504381
Info:   69nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 p.R288Q, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505034
Info:   73nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 WT for p.R288Q, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505035
Info:   73nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 p.N322S, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505874
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EZH2 WT for p.N322S, Human
enhancer of zeste homolog 2 (Drosophila)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505875
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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