Gene:  ETFA, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric lactic ethylmalonic butyric isobutyric 2-methyl-butyric and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

PrimePCR™ SYBR® Green Assay: ETFA, Human
electron-transfer-flavoprotein, alpha polypeptide

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044959
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052221
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PrimePCR™ SYBR® Green Assay: ETFA, Human
electron-transfer-flavoprotein, alpha polypeptide

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015469
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PrimePCR™ Probe Assay: ETFA, Human
electron-transfer-flavoprotein, alpha polypeptide

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0052221
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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