Gene:  ESPL1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1 or separase which initiates the final separation of sister chromatids (Sun et al. 2009 [PubMed 19345191]).[supplied by OMIM Nov 2010]

PrimePCR™ SYBR® Green Assay: ESPL1, Human
extra spindle pole bodies homolog 1 (S. cerevisiae)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0021296
Info:   Same primer pair as used in probe assay qHsaCIP0032992
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PrimePCR™ Probe Assay: ESPL1, Human
extra spindle pole bodies homolog 1 (S. cerevisiae)

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0032992
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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