Gene:  EP300, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha) and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: EP300, Human
E1A binding protein p300

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042782
Info:   Same primer pair as used in probe assay qHsaCEP0050044
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PrimePCR™ SYBR® Green Assay: EP300, Human
E1A binding protein p300

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0016500
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PrimePCR™ Probe Assay: EP300, Human
E1A binding protein p300

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050044
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PrimePCR™ ddPCR™ Mutation Assay:EP300 p.D1399N, Human
E1A binding protein p300

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502910
Info:   73nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EP300 WT for p.D1399N, Human
E1A binding protein p300

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502911
Info:   73nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EP300 p.R1627W, Human
E1A binding protein p300

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505880
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EP300 WT for p.R1627W, Human
E1A binding protein p300

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505881
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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