Gene:  EIF2AK2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1 which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Oct 2011]

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PrimePCR™ SYBR® Green Assay: EIF2AK2, Human
eukaryotic translation initiation factor 2-alpha kinase 2

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042156
Info:   Same primer pair as used in probe assay qHsaCEP0049419
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PrimePCR™ SYBR® Green Assay: EIF2AK2, Human
eukaryotic translation initiation factor 2-alpha kinase 2

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0007581
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: EIF2AK2, Human
eukaryotic translation initiation factor 2-alpha kinase 2

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049419
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: EIF2AK2, Human
eukaryotic translation initiation factor 2-alpha kinase 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5007113
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0042156; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: EIF2AK2, Human
eukaryotic translation initiation factor 2-alpha kinase 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5015199
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0007581; Intron-spanning
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PrimePCR™ ddPCR™ Copy Number Assay: EIF2AK2, Human
eukaryotic translation initiation factor 2-alpha kinase 2

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500392
Info:   FAM; 70nt amplicon; exonic; FAM version of EIF2AK2 (dHsaCP2506588)
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Copy Number Assay: EIF2AK2, Human
eukaryotic translation initiation factor 2-alpha kinase 2

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506588
Info:   HEX; 70nt amplicon; exonic; HEX version of EIF2AK2 (dHsaCP2500392)
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Expression Probe Assay: EIF2AK2, Human
eukaryotic translation initiation factor 2-alpha kinase 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5032538
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0049419; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: EIF2AK2, Human
eukaryotic translation initiation factor 2-alpha kinase 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5032539
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0049419; exonic
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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