Gene:  EGFR, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq Jul 2010]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: EGFR, Human
epidermal growth factor receptor

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0045334
Info:   Same primer pair as used in probe assay qHsaCEP0052595
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PrimePCR™ SYBR® Green Assay: EGFR, Human
epidermal growth factor receptor

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0007564
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: EGFR, Human
epidermal growth factor receptor

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0052595
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PrimePCR™ ddPCR™ Copy Number Assay: EGFR, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000020
Info:   88nt amplicon; intronic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.T790M, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000019
Info:   80nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.T790M, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000020
Info:   80nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.L858R, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000021
Info:   73nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.L858R, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000022
Info:   73nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.E746_A750del, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000039
Info:   78nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.E746_A750del, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000040
Info:   78nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.G719S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000041
Info:   65nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.G719S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000042
Info:   65nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.L861Q, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000043
Info:   64nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.L861Q, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000044
Info:   64nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Copy Number Assay: EGFR, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500318
Info:   64nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.L747_P753>S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500546
Info:   84nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.L747_P753>S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500547
Info:   84nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.R831H, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500602
Info:   63nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.R831H, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500603
Info:   63nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.H773L, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501132
Info:   97nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.H773L, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501133
Info:   97nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.R108K, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501196
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.R108K, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501197
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.G721S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501620
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.G721S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501621
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.G598V, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502140
Info:   69nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.G598V, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502141
Info:   69nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.T847I, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502536
Info:   60nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.T847I, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502537
Info:   60nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.G724S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502932
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.G724S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502933
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.G719A, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502974
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.G719A, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502975
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.R677H, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503102
Info:   64nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.R677H, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503103
Info:   64nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.E709G, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503536
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.E709G, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503537
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.E709K, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503578
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.E709K, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503579
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.L833V, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503904
Info:   63nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.L833V, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503905
Info:   63nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.V765M, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503938
Info:   64nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.V765M, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503939
Info:   64nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.L703P, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503956
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.L703P, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503957
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.G863D, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504076
Info:   64nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.G863D, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504077
Info:   64nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.T751I, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504122
Info:   78nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.T751I, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504123
Info:   78nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.S720F, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504126
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.S720F, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504127
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.G719C, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504192
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.G719C, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504193
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.S784P, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504408
Info:   62nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.S784P, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504409
Info:   62nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.A839T, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504556
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.A839T, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504557
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.S492R, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504568
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.S492R, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504569
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.G735S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504606
Info:   98nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.G735S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504607
Info:   98nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.H835L, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504836
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.H835L, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504837
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.D761Y, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504888
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.D761Y, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504889
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.R836C, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505046
Info:   66nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.R836C, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505047
Info:   66nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.L814P, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505144
Info:   79nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.L814P, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505145
Info:   79nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.V843I, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505160
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.V843I, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505161
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.A289V, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505220
Info:   57nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.A289V, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505221
Info:   57nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.G719D, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505552
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.G719D, Human
epidermal growth factor receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505553
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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