Gene:  EGFR, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq Jul 2010]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: EGFR, Human
epidermal growth factor receptor

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0045334
Info:   Same primer pair as used in probe assay qHsaCEP0052595
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PrimePCR™ SYBR® Green Assay: EGFR, Human
epidermal growth factor receptor

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0007564
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: EGFR, Human
epidermal growth factor receptor

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0052595
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: EGFR, Human
epidermal growth factor receptor

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5009885
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0045334; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: EGFR, Human
epidermal growth factor receptor

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5015184
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0007564; Intron-spanning
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PrimePCR™ ddPCR™ Copy Number Assay: EGFR, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000020
Info:   FAM: 88nt amplicon; intronic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.T790M, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000019
Info:   80nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.T790M, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000020
Info:   80nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.L858R, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000021
Info:   73nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.L858R, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000022
Info:   73nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.E746_A750del, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000039
Info:   78nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.E746_A750del, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000040
Info:   78nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.G719S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000041
Info:   65nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.G719S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000042
Info:   65nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.L861Q, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000043
Info:   64nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.L861Q, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000044
Info:   64nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Copy Number Assay: EGFR, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500318
Info:   FAM; 64nt amplicon; exonic; FAM version of EGFR (dHsaCP2506502)
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.L747_P753>S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500546
Info:   84nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.L747_P753>S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500547
Info:   84nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Copy Number Assay: EGFR, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506502
Info:   HEX; 64nt amplicon; exonic; HEX version of EGFR (dHsaCP2500318)
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.L747_A750>P, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506748
Info:   84nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.L747_A750>P, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506749
Info:   84nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.L747_T751delLREAT, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506752
Info:   84nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.L747_T751delLREAT, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506753
Info:   84nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.L747_S752delLREATS, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506758
Info:   84nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.L747_S752delLREATS, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506759
Info:   84nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.E709K, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506792
Info:   70nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.E709K, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506793
Info:   70nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.E709A, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506858
Info:   70nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.E709A, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506859
Info:   70nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.L747_T751>P, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506890
Info:   84nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.L747_T751>P, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506891
Info:   84nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: EGFR p.S768I, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506892
Info:   80nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: EGFR WT for p.S768I, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506893
Info:   80nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Copy Number Assay: EGFR, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2507032
Info:   HEX; 88nt amplicon; intronic; HEX version of EGFR (dHsaCP1000020)
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Expression Probe Assay: EGFR, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5038080
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0052595; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: EGFR, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5038081
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0052595; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.R831H, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500602
Info:   63nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.R831H, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500603
Info:   63nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.H773L, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501132
Info:   97nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.H773L, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501133
Info:   97nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.R108K, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501196
Info:   70nt amplicon; exonic; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.R108K, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501197
Info:   70nt amplicon; exonic; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.G721S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501620
Info:   80nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.G721S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501621
Info:   80nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.G598V, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502140
Info:   69nt amplicon; exonic; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.G598V, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502141
Info:   69nt amplicon; exonic; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.T847I, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502536
Info:   60nt amplicon; exonic; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.T847I, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502537
Info:   60nt amplicon; exonic; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.G724S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502932
Info:   65nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.G724S, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502933
Info:   65nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.G719A, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502974
Info:   65nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.G719A, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502975
Info:   65nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.R677H, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503102
Info:   64nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.R677H, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503103
Info:   64nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.E709G, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503536
Info:   70nt amplicon; exonic; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.E709G, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503537
Info:   70nt amplicon; exonic; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.E709K, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503578
Info:   70nt amplicon; exonic; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.E709K, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503579
Info:   70nt amplicon; exonic; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.L833V, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503904
Info:   63nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.L833V, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503905
Info:   63nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.V765M, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503938
Info:   64nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.V765M, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503939
Info:   64nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.L703P, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503956
Info:   65nt amplicon; exonic; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.L703P, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503957
Info:   65nt amplicon; exonic; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.G863D, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504076
Info:   64nt amplicon; exonic; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.G863D, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504077
Info:   64nt amplicon; exonic; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.T751I, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504122
Info:   78nt amplicon; exonic; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.T751I, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504123
Info:   78nt amplicon; exonic; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.S720F, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504126
Info:   65nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.S720F, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504127
Info:   65nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.G719C, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504192
Info:   65nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.G719C, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504193
Info:   65nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EGFR p.S784P, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504408
Info:   62nt amplicon; exonic; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EGFR WT for p.S784P, Human
epidermal growth factor receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504409
Info:   62nt amplicon; exonic; in silico
 
Fluorophore:   HEX
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