Gene:  E2F5, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP) a transactivation domain enriched in acidic amino acids and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107 but not with pRB. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: E2F5, Human
E2F transcription factor 5, p130-binding

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044507
Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0051769
List Price:    $130.00
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PrimePCR™ SYBR® Green Assay: E2F5, Human
E2F transcription factor 5, p130-binding

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0007309
List Price:    $130.00
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PrimePCR™ Probe Assay: E2F5, Human
E2F transcription factor 5, p130-binding

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051769
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: E2F5, Human
E2F transcription factor 5, p130-binding

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5009148
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0044507; exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: E2F5, Human
E2F transcription factor 5, p130-binding

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5014984
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0007309; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: E2F5, Human
E2F transcription factor 5, p130-binding

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5036608
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0051769; exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: E2F5, Human
E2F transcription factor 5, p130-binding

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5036609
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0051769; exonic
 
Fluorophore:   HEX
List Price:    $195.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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