Gene:  E2F1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP) a transactivation domain enriched in acidic amino acids and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members E2F2 and E2F3 have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: E2F1, Human
E2F transcription factor 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042864
Info:   Same primer pair as used in probe assay qHsaCEP0050126
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PrimePCR™ SYBR® Green Assay: E2F1, Human
E2F transcription factor 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0016226
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PrimePCR™ Probe Assay: E2F1, Human
E2F transcription factor 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050126
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PrimePCR™ ddPCR™ Copy Number Assay: E2F1, Human
E2F transcription factor 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000299
 
Fluorophore:   FAM
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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