Gene:  DMPK, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin the beta-subunit of the L-type calcium channels and phospholemman. The 3' untranslated region of this gene contains 5-37 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5000 copies causes myotonic dystrophy type I which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region. Several alternatively spliced transcript variants of this gene have been described but the full-length nature of some of these variants has not been determined. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: DMPK, Human
dystrophia myotonica-protein kinase

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0014430
Info:   Same primer pair as used in probe assay qHsaCIP0029376
List Price:    $130.00
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PrimePCR™ Probe Assay: DMPK, Human
dystrophia myotonica-protein kinase

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0029376
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: DMPK, Human
dystrophia myotonica-protein kinase

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5020052
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0014430; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: DMPK, Human
dystrophia myotonica-protein kinase

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5052236
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0029376; Intron-spanning
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: DMPK, Human
dystrophia myotonica-protein kinase

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5052237
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0029376; Intron-spanning
 
Fluorophore:   HEX
List Price:    $195.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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