Gene:  DDX3X, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation nuclear and mitochondrial splicing and ribosome and spliceosome assembly. Based on their distribution patterns some members of this family are believed to be involved in embryogenesis spermatogenesis and cellular growth and division. This gene encodes a DEAD box protein which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. Alternative splicing results in multiple transcript variants.[provided by RefSeq Jul 2010]

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PrimePCR™ SYBR® Green Assay: DDX3X, Human
DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015064
Info:   Same primer pair as used in probe assay qHsaCIP0029684
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PrimePCR™ Probe Assay: DDX3X, Human
DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0029684
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PrimePCR™ ddPCR™ Mutation Assay:DDX3X p.R548M, Human
DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504868
Info:   73nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:DDX3X WT for p.R548M, Human
DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504869
Info:   73nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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