Gene:  CYP27B1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha25-dihydroxyvitamin D3 the active form of vitamin D3 which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: CYP27B1, Human
cytochrome P450, family 27, subfamily B, polypeptide 1

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0003715
Info:   Same primer pair as used in probe assay qHsaCEP0024804
List Price:    $130.00
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PrimePCR™ Probe Assay: CYP27B1, Human
cytochrome P450, family 27, subfamily B, polypeptide 1

Assay Type: Probe
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0024804
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CYP27B1, Human
cytochrome P450, family 27, subfamily B, polypeptide 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5002111
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0003715; Exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CYP27B1, Human
cytochrome P450, family 27, subfamily B, polypeptide 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5026646
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0024804; Exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CYP27B1, Human
cytochrome P450, family 27, subfamily B, polypeptide 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5026647
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0024804; Exonic
 
Fluorophore:   HEX
List Price:    $195.00
Your Price:   Log In
Quantity:  
 
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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