Gene:  CSF1R, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is the receptor for colony stimulating factor 1 a cytokine which controls the production differentiation and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: CSF1R, Human
colony stimulating factor 1 receptor

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0047538
Info:   Same primer pair as used in probe assay qHsaCEP0054798
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PrimePCR™ SYBR® Green Assay: CSF1R, Human
colony stimulating factor 1 receptor

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010604
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PrimePCR™ Probe Assay: CSF1R, Human
colony stimulating factor 1 receptor

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0054798
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CSF1R, Human
colony stimulating factor 1 receptor

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5011862
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0047538; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CSF1R, Human
colony stimulating factor 1 receptor

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5017241
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0010604; Intron-spanning
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PrimePCR™ ddPCR™ Copy Number Assay: CSF1R, Human
colony stimulating factor 1 receptor

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000495
Info:   FAM; 64nt amplicon; crosses exon-intron junction; FAM version of CSF1R (dHsaCP2506338)
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Copy Number Assay: CSF1R, Human
colony stimulating factor 1 receptor

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506338
Info:   HEX; 64nt amplicon; crosses exon-intron junction; HEX version of CSF1R (dHsaCP1000495)
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Expression Probe Assay: CSF1R, Human
colony stimulating factor 1 receptor

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5042034
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0054798; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: CSF1R, Human
colony stimulating factor 1 receptor

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5042035
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0054798; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:CSF1R p.Y969C, Human
colony stimulating factor 1 receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501096
Info:   64nt amplicon; exonic; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CSF1R WT for p.Y969C, Human
colony stimulating factor 1 receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501097
Info:   64nt amplicon; exonic; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:CSF1R p.L301S, Human
colony stimulating factor 1 receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502504
Info:   62nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CSF1R WT for p.L301S, Human
colony stimulating factor 1 receptor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502505
Info:   62nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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