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Bio-Rad collaborated with Biogazelle, leaders in real-time PCR research, to design and experimentally validate PCR primers for gene expression assays across the human and mouse transcriptomes. All PCR primers were designed to meet stringent performance standards following the MIQE guidelines (minimum information for publication of quantitative real-time PCR experiments; Bustin et al. 2009).
Assay Performance Standards
These DNA primer pairs were designed by prioritizing the gene regions most commonly found in transcript variants. Strict design criteria were used to ensure optimal real-time PCR results for each target:
Every PCR primer pair was experimentally validated using Bio-Rad’s iScript™ advanced cDNA synthesis kit and SsoAdvanced™ SYBR® Green supermix. PrimePCR assay design and validation are fully described in the following publication.
PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-lab Validation
Thomson Reuters provided interactive pathway maps for 260 canonical pathways. Each pathway belongs to one or more general biological categories such as cancer. The pathway maps illustrate protein interactions and regulation to provide a comprehensive picture of signaling and disease processes. The selected pathways were used to design panels of real-time PCR primers tailored for the top-ranked genes for differential gene expression analysis. Each gene target within a pathway was assigned a score based on the frequency of differential expression and its research significance. The resulting scores were used to select the assays included in the corresponding real-time PCR pathway panel.
PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy
Control assays and synthetic DNA templates were designed to facilitate the assessment of the key experimental factors impacting your real-time PCR results.
DNA Contamination Control AssayUse the PrimePCR DNA contamination control assay to determine if genomic DNA (gDNA) is present in a sample at a level that may affect PCR results. This assay may also be used to compare relative levels of gDNA contamination present in different samples to determine if PCR results may be affected.
Positive PCR Control AssayUse the PrimePCR positive control assay to qualitatively assess the performance of a PCR reaction associated with a single sample. This assay may also be used to compare the relative performance of PCR reactions associated with different samples.
RNA Quality AssayUse the PrimePCR RNA quality assay to determine if RNA integrity may adversely affect PCR results for a single sample. This assay may also be used to compare relative RNA integrity among different samples to determine how PCR results might be affected.
Reverse Transcription Control AssayUse the PrimePCR reverse transcription control assay to qualitatively assess the performance of the reverse transcription reaction associated with a single sample. This assay may also be used to compare the relative performance of the reverse transcription reactions associated with different samples.
Reference Gene AssaysReference genes are used in relative gene expression analysis to normalize for variation in the amount of input messenger RNA (mRNA) among samples. To ensure accurate quantitation, it is important to include one or more reference genes exhibiting constant expression levels under the experimental conditions. To streamline reference gene selection, we offer PCR primers for a set of commonly used reference genes that can be used individually, easily screened using our preplated 96-well and 384-well reference panels or added to custom-designed plates.
Show Reference Gene AssaysView Reference Gene Assay Panels
Crystallins are separated into two classes: taxon-specific or enzyme and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development these crystallins are made and then retained throughout life making them extremely stable proteins. Mammalian lens crystallins are divided into alpha beta and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs a connecting peptide and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B for acidic and basic respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. [provided by RefSeq Jul 2008]
PrimePCR™ PreAmp for SYBR® Green Assay: CRYAB, Human
PrimePCR™ Template for SYBR® Green Assay: CRYAB, Human
PrimePCR™ PreAmp for Probe Assay: CRYAB, Human
PrimePCR™ Template for Probe Assay: CRYAB, Human
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Aurum™ Total RNA Mini KitThe Aurum total RNA mini kit produces high-quality DNA-free total RNA from a wide range of starting materials including cultured cells, bacteria, and yeast, as well as animal and plant tissues.
iScript™ Advanced cDNA Synthesis Kit for RT-qPCRThe iScript advanced cDNA synthesis kit for RT-qPCR is a simple, fast, and sensitive first-strand cDNA synthesis kit for gene expression analysis by reverse-transcription qPCR.
PrimePCR™ Assays and PanelsExperimentally validated real-time PCR assays are available as individual assays, preplated pathway- and disease-specific panels, or custom- configured plates.
SsoAdvanced™ Universal Real-Time PCR SupermixThe SsoAdvanced™ universal real-time PCR supermixes employ our patented Sso7d fusion enzyme technology for higher processivity, increased PCR inhibitor tolerance, and robust performance with difficult qPCR targets. Available for probe- and dye-based assays in ROX- dependent or ROX-independent real-time PCR detection systems.
Real-Time PCR Detection SystemsReal-time PCR amplification systems combine thermal cyclers with optical reaction modules for singleplex and multiplex detection of fluorophores. All systems feature thermal gradient functionality.
Amplification SoftwareSoftware for Bio-Rad thermal cyclers supports multi-instrument control and collection and analysis of real-time data. Data analysis tools include ΔCq and ΔΔCq analyses and scatter plot, volcano plot, heat map, and clustergram outputs.
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View the products required to complete your real-time PCR experiments.
Pricing for PrimePCR real-time PCR assays is based on assay type, the number of reactions, and plate configuration for 96- and 384-well plates.
Discount applies to bulk orders of the same catalog number and is taken off the list price.
Bulk discounts are shown below; download the pricing flyer for full details.
Real-Time PCR Assays and Controls
5 or more assays
Pathway and Collection Panels
20 or more plates
Custom PlatesCustom 96-well & 384-well plates with 1–96 unique genes
Custom 384-well plates with 97 or more unique genes
30 or more plates
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