Gene:  COMT, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines including the neurotransmitters dopamine epinephrine and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances COMT is important in the metabolism of catechol drugs used in the treatment of hypertension asthma and Parkinson disease. COMT is found in two forms in tissues a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq Sep 2008]

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PrimePCR™ SYBR® Green Assay: COMT, Human
catechol-O-methyltransferase

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0001845
Info:   Recommended - best coverage
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PrimePCR™ SYBR® Green Assay: COMT, Human
catechol-O-methyltransferase

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042669
Info:   Same primer pair as used in probe assay qHsaCEP0049931
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PrimePCR™ Probe Assay: COMT, Human
catechol-O-methyltransferase

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049931
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: COMT, Human
catechol-O-methyltransferase

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5000685
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0001845; Exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: COMT, Human
catechol-O-methyltransferase

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5007522
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0042669; exonic
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PrimePCR™ ddPCR™ Expression Probe Assay: COMT, Human
catechol-O-methyltransferase

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5033356
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0049931; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: COMT, Human
catechol-O-methyltransferase

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5033357
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0049931; exonic
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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