Gene:  COL4A4, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes one of the six subunits of type IV collagen the major structural component of basement membranes. This particular collagen IV subunit however is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts differing only in their transcription start sites have been identified for this gene and as is common for collagen genes multiple polyadenylation sites are found in the 3' UTR. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: COL4A4, Human
collagen, type IV, alpha 4

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0016411
Info:   Same primer pair as used in probe assay qHsaCIP0030571
List Price:    $130.00
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PrimePCR™ Probe Assay: COL4A4, Human
collagen, type IV, alpha 4

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0030571
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: COL4A4, Human
collagen, type IV, alpha 4

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5021646
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0016411; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: COL4A4, Human
collagen, type IV, alpha 4

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5054424
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0030571; Intron-spanning
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: COL4A4, Human
collagen, type IV, alpha 4

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5054425
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0030571; Intron-spanning
 
Fluorophore:   HEX
List Price:    $195.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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