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Thomson Reuters provided interactive pathway maps for 260 canonical pathways. Each pathway belongs to one or more general biological categories such as cancer. The pathway maps illustrate protein interactions and regulation to provide a comprehensive picture of signaling and disease processes. The selected pathways were used to design panels of real-time PCR primers tailored for the top-ranked genes for differential gene expression analysis. Each gene target within a pathway was assigned a score based on the frequency of differential expression and its research significance. The resulting scores were used to select the assays included in the corresponding real-time PCR pathway panel.
PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy
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PrimePCR™ PreAmp for SYBR® Green Assay: CHST9, Chicken
PrimePCR™ Template for SYBR® Green Assay: CHST9, Chicken
PrimePCR™ PreAmp for Probe Assay: CHST9, Chicken
PrimePCR™ Template for Probe Assay: CHST9, Chicken