Gene:  CDK6, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28 and Schizosaccharomyces pombe cdc2 and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase as well as CDK4 has been shown to phosphorylate and thus regulate the activity of tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Nov 2009]

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PrimePCR™ SYBR® Green Assay: CDK6, Human
cyclin-dependent kinase 6

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043099
Info:   Same primer pair as used in probe assay qHsaCEP0050361
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PrimePCR™ SYBR® Green Assay: CDK6, Human
cyclin-dependent kinase 6

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010553
Info:   Recommended - best efficiency
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PrimePCR™ Probe Assay: CDK6, Human
cyclin-dependent kinase 6

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050361
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PrimePCR™ ddPCR™ Copy Number Assay: CDK6, Human
cyclin-dependent kinase 6

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000014
Info:   75nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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