Gene:  CDH1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric breast colorectal thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation invasion and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: CDH1, Human
cadherin 1, type 1, E-cadherin (epithelial)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042076
Info:   Same primer pair as used in probe assay qHsaCEP0049339
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PrimePCR™ SYBR® Green Assay: CDH1, Human
cadherin 1, type 1, E-cadherin (epithelial)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015365
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: CDH1, Human
cadherin 1, type 1, E-cadherin (epithelial)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049339
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PrimePCR™ ddPCR™ Copy Number Assay: CDH1, Human
cadherin 1, type 1, E-cadherin (epithelial)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000585
Info:   67nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CDH1 p.A592T, Human
cadherin 1, type 1, E-cadherin (epithelial)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501566
Info:   67nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CDH1 WT for p.A592T, Human
cadherin 1, type 1, E-cadherin (epithelial)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501567
Info:   67nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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