Gene:  CDC45, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45 an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18 the minichromosome maintenance proteins (MCMs) and DNA polymerase which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq May 2010]

PrimePCR™ SYBR® Green Assay: CDC45, Human
cell division cycle 45 homolog (S. cerevisiae)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0036357
Info:   Same primer pair as used in probe assay qHsaCEP0039081
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PrimePCR™ Probe Assay: CDC45, Human
cell division cycle 45 homolog (S. cerevisiae)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0039081
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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