Gene:  CD3E, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is the CD3-epsilon polypeptide which together with CD3-gamma -delta and -zeta and the T-cell receptor alpha/beta and gamma/delta heterodimers forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: CD3E, Human
CD3e molecule, epsilon (CD3-TCR complex)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0048386
Info:   Same primer pair as used in probe assay qHsaCEP0055646
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PrimePCR™ SYBR® Green Assay: CD3E, Human
CD3e molecule, epsilon (CD3-TCR complex)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0011826
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PrimePCR™ Probe Assay: CD3E, Human
CD3e molecule, epsilon (CD3-TCR complex)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0055646
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CD3E, Human
CD3e molecule, epsilon (CD3-TCR complex)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5012621
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0048386; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CD3E, Human
CD3e molecule, epsilon (CD3-TCR complex)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5018175
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0011826; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: CD3E, Human
CD3e molecule, epsilon (CD3-TCR complex)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5043552
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0055646; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: CD3E, Human
CD3e molecule, epsilon (CD3-TCR complex)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5043553
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0055646; exonic
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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