Gene:  CBL, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq Mar 2012]

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PrimePCR™ SYBR® Green Assay: CBL, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043313
Info:   Same primer pair as used in probe assay qHsaCEP0050575
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PrimePCR™ SYBR® Green Assay: CBL, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0016418
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PrimePCR™ Probe Assay: CBL, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050575
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PrimePCR™ ddPCR™ Copy Number Assay: CBL, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000516
Info:   68nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CBL p.Y371H, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500954
Info:   97nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CBL WT for p.Y371H, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500955
Info:   97nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:CBL p.C396R, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501020
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CBL WT for p.C396R, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501021
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:CBL p.C404Y, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501586
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CBL WT for p.C404Y, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501587
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:CBL p.L380P, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501592
Info:   115nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CBL WT for p.L380P, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501593
Info:   115nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:CBL p.C384Y, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504264
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CBL WT for p.C384Y, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504265
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:CBL p.R420Q, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504522
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CBL WT for p.R420Q, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504523
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:CBL p.F418S, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505228
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CBL WT for p.F418S, Human
Cas-Br-M (murine) ecotropic retroviral transforming sequence

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505229
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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