Gene:  CASP10, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits large and small that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7 and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Apr 2011]

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PrimePCR™ SYBR® Green Assay: CASP10, Human
caspase 10, apoptosis-related cysteine peptidase

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0009223
Info:   Same primer pair as used in probe assay qHsaCIP0027098
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PrimePCR™ Probe Assay: CASP10, Human
caspase 10, apoptosis-related cysteine peptidase

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0027098
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PrimePCR™ ddPCR™ Mutation Assay:CASP10 p.T328T, Human
caspase 10, apoptosis-related cysteine peptidase

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503268
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CASP10 WT for p.T328T, Human
caspase 10, apoptosis-related cysteine peptidase

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503269
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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