Gene:  C4A, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes the acidic form of complement factor 4 part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha beta and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist such that individuals may have 1 2 or 3 copies of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Nov 2011]

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PrimePCR™ SYBR® Green Assay: C4A, Human
complement component 4A (Rodgers blood group)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0037519
Info:   Same primer pair as used in probe assay qHsaCEP0040242
List Price:    $125.00
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PrimePCR™ Probe Assay: C4A, Human
complement component 4A (Rodgers blood group)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0040242
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: C4A, Human
complement component 4A (Rodgers blood group)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5188615
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0037519; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: C4A, Human
complement component 4A (Rodgers blood group)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5190921
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0040242; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: C4A, Human
complement component 4A (Rodgers blood group)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5190922
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0040242; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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