Gene:  BPTF, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene was identified by the reactivity of its encoded protein to a monoclonal antibody prepared against brain homogenates from patients with Alzheimer's disease. Analysis of the original protein (fetal Alz-50 reactive clone 1 or FAC1) identified as an 810 aa protein containing a DNA-binding domain and a zinc finger motif suggested it might play a role in the regulation of transcription. High levels of FAC1 were detected in fetal brain and in patients with neurodegenerative diseases. The protein encoded by this gene is actually much larger than originally thought and it also contains a C-terminal bromodomain characteristic of proteins that regulate transcription during proliferation. The encoded protein is highly similar to the largest subunit of the Drosophila NURF (nucleosome remodeling factor) complex. In Drosophila the NURF complex which catalyzes nucleosome sliding on DNA and interacts with sequence-specific transcription factors is necessary for the chromatin remodeling required for transcription. Two alternative transcripts encoding different isoforms have been described completely. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: BPTF, Human
bromodomain PHD finger transcription factor

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0046828
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054089
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: BPTF, Human
bromodomain PHD finger transcription factor

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0007347
List Price:    $125.00
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PrimePCR™ Probe Assay: BPTF, Human
bromodomain PHD finger transcription factor

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0054089
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: BPTF, Human
bromodomain PHD finger transcription factor

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5011225
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0046828; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: BPTF, Human
bromodomain PHD finger transcription factor

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5015016
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0007347; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: BPTF, Human
bromodomain PHD finger transcription factor

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5040760
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0054089; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: BPTF, Human
bromodomain PHD finger transcription factor

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5040761
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0054089; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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