Gene:  ATP6V0E1, Human

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This gene encodes a component of vacuolar ATPase (V-ATPase) a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting zymogen activation receptor-mediated endocytosis and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits two G subunits plus the C D E F and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a c c' c" and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is possibly part of the V0 subunit. Since two nontranscribed pseudogenes have been found in dog it is possible that the localization to chromosome 2 for this gene by radiation hybrid mapping is representing a pseudogene. Genomic mapping puts the chromosomal location on 5q35.3. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: ATP6V0E1, Human
ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043516
Info:   Same primer pair as used in probe assay qHsaCEP0050778
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: ATP6V0E1, Human
ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0014535
List Price:    $125.00
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PrimePCR™ Probe Assay: ATP6V0E1, Human
ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050778
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: ATP6V0E1, Human
ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5008281
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0043516; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: ATP6V0E1, Human
ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5020132
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0014535; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ATP6V0E1, Human
ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5034874
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0050778; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ATP6V0E1, Human
ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5034875
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0050778; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
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LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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