Gene:  Apob, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human which are synthesized exclusively in the gut and liver respectively the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA) resulting in the creation of a stop codon and early translation termination. [provided by RefSeq Jul 2008]

PrimePCR™ SYBR® Green Assay: Apob, Mouse
Apolipoprotein B

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qMmuCED0044141
Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0052567
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PrimePCR™ SYBR® Green Assay: Apob, Mouse
Apolipoprotein B

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qMmuCID0025252
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PrimePCR™ Probe Assay: Apob, Mouse
Apolipoprotein B

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qMmuCEP0052567
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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