Gene:  APOB, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA) resulting in the creation of a stop codon and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia normotriglyceridemic hypobetalipoproteinemia and hypercholesterolemia due to ligand-defective apoB diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: APOB, Human
apolipoprotein B (including Ag(x) antigen)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0016796
Info:   Same primer pair as used in probe assay qHsaCIP0030812
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PrimePCR™ Probe Assay: APOB, Human
apolipoprotein B (including Ag(x) antigen)

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0030812
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PrimePCR™ ddPCR™ Mutation Assay:APOB p.L1527P, Human
apolipoprotein B (including Ag(x) antigen)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502404
Info:   80nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APOB WT for p.L1527P, Human
apolipoprotein B (including Ag(x) antigen)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502405
Info:   80nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APOB p.F3102L, Human
apolipoprotein B (including Ag(x) antigen)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504440
Info:   69nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APOB WT for p.F3102L, Human
apolipoprotein B (including Ag(x) antigen)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504441
Info:   69nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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