Gene:  APEX1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq Jul 2008]

PrimePCR™ SYBR® Green Assay: APEX1, Human
APEX nuclease (multifunctional DNA repair enzyme) 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0012533
Info:   Same primer pair as used in probe assay qHsaCIP0028481
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PrimePCR™ Probe Assay: APEX1, Human
APEX nuclease (multifunctional DNA repair enzyme) 1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028481
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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