Gene:  APC, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion transcriptional activation and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP) an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: APC, Human
adenomatous polyposis coli

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0012226
Info:   Same primer pair as used in probe assay qHsaCIP0028348
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PrimePCR™ Probe Assay: APC, Human
adenomatous polyposis coli

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028348
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PrimePCR™ ddPCR™ Copy Number Assay: APC, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500356
Info:   66nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: APC p.R1450*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500508
Info:   63nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: APC WT for p.R1450*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500509
Info:   63nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.E1286*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500676
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.E1286*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500677
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.E1379*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500752
Info:   79nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.E1379*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500753
Info:   79nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.E1451*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500794
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.E1451*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500795
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.T1537K, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500952
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.T1537K, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500953
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.S811*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501054
Info:   95nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.S811*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501055
Info:   95nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.S1356*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501062
Info:   94nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.S1356*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501063
Info:   94nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.R332*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501100
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.R332*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501101
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.Y1376*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501112
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.Y1376*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501113
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.Q1469*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501156
Info:   68nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.Q1469*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501157
Info:   68nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.E1317E, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501160
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.E1317E, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501161
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.E1464*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501284
Info:   79nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.E1464*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501285
Info:   79nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.R216*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501334
Info:   120nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.R216*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501335
Info:   120nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.R232*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501386
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.R232*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501387
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.K1454E, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501444
Info:   79nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.K1454E, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501445
Info:   79nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.R283*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501652
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.R283*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501653
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.S1400*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501698
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.S1400*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501699
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.E1536*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501702
Info:   67nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.E1536*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501703
Info:   67nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.Y935*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501730
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.Y935*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501731
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.K1310*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501742
Info:   74nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.K1310*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501743
Info:   74nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.S1346*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501832
Info:   67nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.S1346*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501833
Info:   67nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.Q1367*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501856
Info:   62nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.Q1367*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501857
Info:   62nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.R1331*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502022
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.R1331*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502023
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.R805*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502026
Info:   78nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.R805*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502027
Info:   78nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.S1315*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502382
Info:   78nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.S1315*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502383
Info:   78nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.Q1378*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502446
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.Q1378*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502447
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.Q1338*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502480
Info:   95nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.Q1338*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502481
Info:   95nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.E1577*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502542
Info:   119nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.E1577*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502543
Info:   119nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.S130G, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502712
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.S130G, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502713
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.E1408*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502796
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.E1408*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502797
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.E1353*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502824
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.E1353*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502825
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.E1374*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502876
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.E1374*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502877
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.G1312*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502978
Info:   66nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.G1312*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502979
Info:   66nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.S1398G, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503120
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.S1398G, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503121
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.S1327*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503236
Info:   62nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.S1327*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503237
Info:   62nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.E918*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503298
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APC WT for p.E918*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503299
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APC p.R405*, Human
adenomatous polyposis coli

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503310
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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