Gene:  ALDOB, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Fructose-16-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-16-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A B and C are distinct proteins the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver kidney and intestine aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq Dec 2008]

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PrimePCR™ SYBR® Green Assay: ALDOB, Human
aldolase B, fructose-bisphosphate

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0012845
Info:   Same primer pair as used in probe assay qHsaCIP0028624
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PrimePCR™ Probe Assay: ALDOB, Human
aldolase B, fructose-bisphosphate

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028624
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PrimePCR™ ddPCR™ Mutation Assay:ALDOB p.K99R, Human
aldolase B, fructose-bisphosphate

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502456
Info:   64nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ALDOB WT for p.K99R, Human
aldolase B, fructose-bisphosphate

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502457
Info:   64nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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