Gene:  Akt1, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes the founding member of the Akt serine-threonine protein kinase gene family that also includes Akt2 and Akt3. This kinase is a major downstream effector of the phosphatidylinositol 3-kinase (PI3K) pathway that mediates the effects of various growth factors such as platelet-derived growth factor (PDGF) epidermal growth factor (EGF) insulin and insulin-like growth factor I (IGF-I). It is activated through recruitment to cellular membranes by PI3K lipid products and by phosphorylation by 3-phosphoinositide dependent kinase-1. It then further phosphorylates different downstream proteins in response to various extracellular signals and thus plays a pivotal role in mediating a variety of cellular processes such as glucose metabolism glycogen biosynthesis protein synthesis and turn over inflammatory response cell survival (anti-apoptosis) and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2009]

PrimePCR™ SYBR® Green Assay: Akt1, Mouse
Thymoma viral proto-oncogene 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qMmuCED0044805
Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0053231
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PrimePCR™ SYBR® Green Assay: Akt1, Mouse
Thymoma viral proto-oncogene 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qMmuCID0023089
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PrimePCR™ Probe Assay: Akt1, Mouse
Thymoma viral proto-oncogene 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qMmuCEP0053231
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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