Gene:  ACTN4, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions where it is involved in binding actin to the membrane. In contrast skeletal cardiac and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle alpha actinin isoform which is concentrated in the cytoplasm and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: ACTN4, Human
actinin, alpha 4

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0012278
Info:   Same primer pair as used in probe assay qHsaCIP0028372
List Price:    $130.00
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PrimePCR™ Probe Assay: ACTN4, Human
actinin, alpha 4

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028372
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: ACTN4, Human
actinin, alpha 4

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5018525
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0012278; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Copy Number Assay: ACTN4, Human
actinin, alpha 4

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000457
Info:   FAM: 69nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
List Price:    $240.00
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PrimePCR™ ddPCR™ Copy Number Assay: ACTN4, Human
actinin, alpha 4

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506956
Info:   HEX; 69nt amplicon; crosses exon-intron junction; HEX version of ACTN4 (dHsaCP1000457)
 
Fluorophore:   HEX
List Price:    $240.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ACTN4, Human
actinin, alpha 4

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5050594
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0028372; Intron-spanning
 
Fluorophore:   FAM
List Price:    $195.00
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Expression Probe Assay: ACTN4, Human
actinin, alpha 4

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5050595
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0028372; Intron-spanning
 
Fluorophore:   HEX
List Price:    $195.00
Your Price:   Log In
Quantity:  
 
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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