Gene:  ACTN1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions where it is involved in binding actin to the membrane. In contrast skeletal cardiac and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle cytoskeletal alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

PrimePCR™ SYBR® Green Assay: ACTN1, Human
actinin, alpha 1

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0002731
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PrimePCR™ SYBR® Green Assay: ACTN1, Human
actinin, alpha 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0056402
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057507
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PrimePCR™ Probe Assay: ACTN1, Human
actinin, alpha 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0057507
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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