Gene:  ABL1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation cell division cell adhesion and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: ABL1, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0006676
Info:   Same primer pair as used in probe assay qHsaCIP0026252
List Price:    $125.00
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PrimePCR™ Probe Assay: ABL1, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0026252
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: ABL1, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5014631
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0006676; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Copy Number Assay: ABL1, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000587
Info:   FAM; 64nt amplicon; crosses exon-intron junction; FAM version of ABL1 (dHsaCP2506490)
 
Fluorophore:   FAM
List Price:    $230.00
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PrimePCR™ ddPCR™ Copy Number Assay: ABL1, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506490
Info:   HEX; 64nt amplicon; crosses exon-intron junction; HEX version of ABL1 (dHsaCP1000587)
 
Fluorophore:   HEX
List Price:    $230.00
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PrimePCR™ ddPCR™ Mutation Assay: ABL1 p.T315I, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506762
Info:   70nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
List Price:    $380.00
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PrimePCR™ ddPCR™ Mutation Assay: ABL1 WT for p.T315I, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506763
Info:   70nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
List Price:    $380.00
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PrimePCR™ ddPCR™ Mutation Assay: ABL1 p.E255K, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506764
Info:   61nt amplicon; exonic
 
Fluorophore:   FAM
List Price:    $380.00
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PrimePCR™ ddPCR™ Mutation Assay: ABL1 WT for p.E255K, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506765
Info:   61nt amplicon; exonic
 
Fluorophore:   HEX
List Price:    $380.00
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PrimePCR™ ddPCR™ Mutation Assay: ABL1 p.Y253H, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506766
Info:   100nt amplicon; exonic
 
Fluorophore:   FAM
List Price:    $380.00
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PrimePCR™ ddPCR™ Mutation Assay: ABL1 WT for p.Y253H, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506767
Info:   100nt amplicon; exonic
 
Fluorophore:   HEX
List Price:    $380.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ABL1, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5046926
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0026252; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ABL1, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5046927
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0026252; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 p.L248V, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501310
Info:   62nt amplicon; exonic; in silico
 
Fluorophore:   FAM
List Price:    $315.00
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 WT for p.L248V, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501311
Info:   62nt amplicon; exonic; in silico
 
Fluorophore:   HEX
List Price:    $315.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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