Gene:  ABL1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation cell division cell adhesion and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: ABL1, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0006676
Info:   Same primer pair as used in probe assay qHsaCIP0026252
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PrimePCR™ Probe Assay: ABL1, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0026252
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PrimePCR™ ddPCR™ Copy Number Assay: ABL1, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000587
Info:   64nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 p.F359V, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500632
Info:   72nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 WT for p.F359V, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500633
Info:   72nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 p.L248V, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501310
Info:   62nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 WT for p.L248V, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501311
Info:   62nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 p.T315I, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501524
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 WT for p.T315I, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501525
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 p.E255K, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502108
Info:   61nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 WT for p.E255K, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502109
Info:   61nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 p.Y253H, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502952
Info:   100nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 WT for p.Y253H, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502953
Info:   100nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 p.M351T, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503572
Info:   67nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 WT for p.M351T, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503573
Info:   67nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 p.F317L, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503940
Info:   62nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 WT for p.F317L, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503941
Info:   62nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 p.G250E, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503962
Info:   63nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 WT for p.G250E, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503963
Info:   63nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 p.Q252H, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503986
Info:   63nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 WT for p.Q252H, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503987
Info:   63nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 p.H396R, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504362
Info:   62nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 WT for p.H396R, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504363
Info:   62nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 p.M244V, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504940
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 WT for p.M244V, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504941
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 p.E355G, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504954
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 WT for p.E355G, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504955
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 p.Y253F, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505676
Info:   100nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ABL1 WT for p.Y253F, Human
c-abl oncogene 1, non-receptor tyrosine kinase

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505677
Info:   100nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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