PrimePCR™ Probe Assay: Pomp, Rat


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0048843
Assay Design:   Intron-spanning
Chromosome Location:   12:11096338-11101183question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000066508 ENSRNOT00000035009 ENSRNOT00000073155 ENSRNOT00000071796 ENSRNOT00000075767 ENSRNOT00000019512 ENSRNOT00000059530 ENSRNOT00000072287 ENSRNOT00000001697 ENSRNOT00000010146 ENSRNOT00000005304 ENSRNOT00000030891 ENSRNOT00000020669 ENSRNOT00000040391 ENSRNOT00000026529 ENSRNOT00000026491 ENSRNOT00000056724 ENSRNOT00000068539 ENSRNOT00000029651 ENSRNOT00000034672 ENSRNOT00000004236 ENSRNOT00000023794 ENSRNOT00000068413 ENSRNOT00000024138 ENSRNOT00000023693 ENSRNOT00000071576 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   Pomp
Gene Name:   Proteasome maturation protein
Aliases:   Not Available
RefSeq:   NM_001100942
Ensembl:   ENSRNOG00000049229
Entrez:   288455
UniGene:   Rn.28242
Chromosome Mapping:   12p11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 37.360000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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